Breakout analyses are useful hatchery management procedures that provide valuable information by helping to relate
problems to the breeder flock, egg handling procedures and/or the hatchery
There are three different procedures for
breakout analysis that can be implemented
by a quality control employee. Each of the
methods has advantages and disadvantages. The data recorded should be used to
continuously control the production procedures and aid in finding room for improvement. If a database has been built up, it can
be used for quick and precise troubleshooting should problems occur.
Fresh egg breakout
The quickest way to estimate the fertility in
a breeder flock is a fresh egg breakout. The
eggs can be cracked open just after lay or
upon arrival at the hatchery. The disadvantages of this breakout method are the loss
of valuable hatching eggs and possible errors of prediction due to the relatively small
sample size (usually 100 eggs per flock). It is
therefore recommended to limit the use of
a fresh egg breakout to situations where a
quick fertility check is required.
• Planning the first setting of eggs from a
young flock.
• Problem flocks.
Infertile and non-incubated fertile eggs can
be differentiated quite precisely after some
practical training. Guidelines and pictures
can be found in the Hatchery Management
Guide of
LOHMANN TIERZUCHT, which is
available upon request.
Candling breakout
The candling breakout analysis offers the
highest accuracy in determining fertility. It is also useful in recording other sources of
breeder flock or egg handling failures such
as, early mortality, cracked eggs or eggs set
upside down. It is therefore a good tool to
monitor the week-to-week status of the
breeder flocks.
Additionally, the candling percentage allows quite a precise prediction of the expected hatch of saleable chicks. As candling is usually done at days 9–10, there is
time to react (e.g. rearrange settings, reschedule shipments and to detect causes
and find solutions to the same) if a high
number of clear eggs is observed. The size
of the sample should be four to six trays of
one flock (at least 600 eggs) from different
locations inside one setter. This sampling
procedure is not only important to avoid
biased results caused by incubation conditions, but also by farm conditions. The eggs
on subsequent trays might be of different
quality than the average of one farm.
Hatch debris breakout
Most often performed in hatcheries is
the breakout on hatch days which is also
known as hatch debris breakout. Although
it gives a full picture about the pattern of
embryo mortality, infertile eggs and those
containing early dead embryos are more
difficult to distinguish. Another disadvantage is the time delay of usually four weeks
(three weeks incubation + one week storage) between the date of lay and the
breakout. A hatch debris breakout should
be done every hatch day or just monthly
in order to build up a database.
LOHMANN TIERZUCHT Hatchery
Management Guide
• To perform a hatch debris breakout,
one should take out four to six hatcher
baskets of one flock from different
positions in one hatcher.
• Remove all unhatched eggs and place
them on pulp trays.
• Record the number of dead chicks and
culls left in the basket.
• Classify the unhatched eggs into
appropriate categories, as Infertile,
Early dead (day 1–7), Mid-dead
(day 8–17), Late dead (>18), Pipped
and Contaminated.
• Additionally, the eggshell should be
assessed and cracked eggs also
recorded.
As already mentioned, the differentiation
between infertile eggs and very early dead
birds is difficult as the blastodisc is often
undetectable. One therefore needs to assess the appearance of the yolk and the
albumen. If the egg still looks like a “table egg” on the day of hatch, it was most
probably infertile. If you deal with very
early dead germs, the colour of the yolk
will change slightly, yolk membrane will
weaken and the albumen will be thinner.
The content of eggs containing embryos
which died during the second week of incubation will often appear black due to the
breakdown of blood. Be careful not to classify these eggs as contaminated as long as
they do not emit an odour. If breakouts are
done regularly with an adequate sample
size, the use of more categories (day 1–2,
day 3–4, Malformation, Malposition, Internally pipped, Pipped alive vs. dead, etc.)
can provide additional information, which
might be overseen when using a more
simple approach. All results from breakout
analyses should be expressed as percentage
of the eggs set, e.g. for three early dead chicks
in a tray of 150 eggs, the result would be
2 %. The breakout data should be analysed
using standards based on the results of the
individual hatchery. From time to time, the
standards should be compared to results
achieved in other hatcheries. If you notice
elevated percentages in single categories, have a look at the appendix of the
LOHMANN TIERZUCHT Hatchery Management Guide where you will find a comprehensive list of probable causes. The
Technical Service Team at
LOHMANN TIERZUCHT is available to assist you with the
set-up of a breakout routine and/or by the
interpretation of the results.
Robert Schulte-Drüggelte
Example of a detailed hatch debris breakout sheet
Troubleshooting and breakout rules:
1. If a problem occurs, check the basic farm, transport and hatchery procedures first.
2. Collect breakout data regularly – also from good hatching flocks – to create your
own database. Keep the records simple enough. You need to work with them.
3. Do not take action just because of the results of one breakout. Check the same
flock again incubated in a different machine or vice-versa.
4. Assure good communication along the production chain, especially between
the breeder farms and hatchery. This may not prevent problems, but significantly
minimise their economic impact.
Embryonic development at day 3 + 10 hours
Embryonic development at day 11 + 10 hours